Recommendations for animal DNA forensic and identity testing

Budowle,B.; Garofano,P.; Hellman,A.; Ketchum,M.; Kanthaswamy,S.; Parson,W.; van Haeringen,W.; Fain,S.; Broad,T.; Genetic analysis in animals has been used for many applications, such as kinship analysis, for determining the sire of an offspring when a female has been exposed to multiple males, determining parentage when an animal switches offspring with another dam, extended lineage reconstruction, estimating inbreeding, identification in breed registries, and speciation. It now also is being used increasingly to characterize animal materials in forensic cases. [Read More]

Separate analysis of DYS385a and b versus conventional DYS385 typing: is there forensic relevance?

Niederstätter,H.; Berger,B.; Oberacher,H.; Brandstätter,A.; Huber,C.G.; Parson,W.; In order to determine to what extent the separate analysis of both copies of DYS385 improves Y-chromosomal short tandem repeat (Y-STR) haplotyping, we followed a recently published protocol for the separate amplification of DYS385a and DYS385b with modifications and compared the results with those obtained by conventional analysis in a population sample comprising 133 unrelated Caucasian males from Austria. Additionally, we typed all markers of the minimal haplotype (minHT) and a set of Y-chromosomal single nucleotide polymorphisms (Y-SNPs) in order to interpret the STR data depending on the Y-SNP haplogroup structure. [Read More]

Unusual variant alleles in commonly used short tandem repeat loci

Grubwieser,P.; Mühlmann,R.; Niederstätter,H.; Pavlic,M.; Parson,W.; Unusually large variant alleles were observed in the short tandem repeat (STR) systems D3S1358 and D21S11, both of which are included in the international standard set of loci (ISSOL) and routinely typed in National DNA intelligence databases worldwide. The observed alleles fell within the size range of the adjacent STR marker, which could easily cause problems with respect to correct allele assignments for both loci concerned. [Read More]