Austrian STR Population Data using the PowerSeq GY46

Austrian STR Population Data using the PowerSeq GY46 System and Massively Parallel Sequencing

Müller,P.; Berger,B.; Bodner,M.; DNASEQEX Consortium; Parson,W.

By using massively parallel sequencing (MPS) technologies to genotype forensic markers, it is possible to characterize sequence variations located in the repeat and flanking regions of short tandem repeats (STRs). To account for sequence based allele frequencies valid for the Austrian population, we performed a population study including 248 unrelated male donors verifiably born in Austria. In accordance with the Austrian law and permission of the Federal Ministry of the Interior, Austria, it is possible to use DNA samples and DNA profiles of the Austrian National DNA Database for population studies, preconditioned the data is made anonymous.

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Evaluation of a costumised panel of 118 novel microhyplotypes

Evaluation of a costumised panel of 118 novel microhyplotypes selected for forensic identification in two MPS platforms

Puente,M.; Phillips,C.; Xavier,C.; Amigo,J.; Carracedo,Á.; Parson,W.; Lareu,M.

Microhaplotypes (MHs) comprise two or more closely-sited SNPs in spans up to 200 nucleoMdes (nt). MH alleles are defined by the phased combinaEons of the alleles of the component SNPs, that can be obtained by Massive Parallel Sequencing (MPS) if the SNPs are co-amplified in one sequence.

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Introducing a forensic version of PhyloTree

Introducing a forensic version of PhyloTree for improved haplogrouping

Dür,A.; Huber,N.; Parson,W.

Mitochondrial DNA haplogrouping is an important tool that is not restricted to forensic mitochondrial genome (mitogenome) analysis. In many population genetic studies, haplogroup determination is a decisive prerequisite for further investigations and research questions.

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Sketching Man’s Best Friend

Sketching Man’s Best Friend - Canine DNA Phenotyping for Forensic Purposes

Heinrich,J.; Berger,B.; Hecht,W.; Hellmann,A.; Rohleder,U.; Schleenbecker,U.; Morf,N.; Phillips,C.; Parson,W.; Berger,C.; CaDNAP Group

Forensic Short Tandem Repeat (STR) analysis is the gold standard to identify canine individuals, or to link crime scene traces to the donor dog. However, forensic DNA fingerprinting loses its evidential power when no canine suspect DNA is available. To overcome this limitation, we aim to develop a method to predict externally visible traits of dogs based on DNA. Previous studies have already shown that externally visible characteristics of dogs are caused by variations in a surprisingly small number of genes and only a few mutations are probably responsible for the extraordinary diversity of this species. Due to this special characteristic of dog genetics, developing a suitable marker set for typing external visible traits appears highly promising and could contribute significantly to the toolbox for canine DNA analysis in forensic casework.

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The Breed Makes the Difference

The Breed Makes the Difference: Pedigree Composition, Canine STR Allele Frequencies, and Random Match Probabilities

Berger,B.; Berger,C.; Heinrich,J.; Hecht,W.; Hellmann,A.; Schleenbecker,U.; Rohleder,U.; Morf,N.; CaDNAP Group; Parson,W.

Domestic dogs are popular and as integral part in everyday life they can become relevant to forensic DNA analysis, which is typically conducted by canine STR analysis followed by assessing the weight of evidence of a matching DNA profile, e.g. by random match probability (RMP) calculations. Noteworthy in this context is the outstanding diversity of dogs. Breeders have exerted selective pressure throughout a long period creating the extraordinary variety of modern dog breeds, unparalleled in any other animal species.

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