Analysis of cannabis seizures by non-targeted liquid chromatography-tandem mass spectrometry

Stempfer,M.; Reinstadler,V.; Lang,A.; Oberacher,H.; Due to the popularity of recreational cannabis use, contamination of this drug with diverse classes of chemicals, including pesticides, mycotoxins, and synthetic cannabinoids, has been identified as major threat for public health. For the detection of these compounds in seized cannabis, a screening workflow involving non-targeted liquid chromatography-tandem mass spectrometry (LCMS/MS) was developed. A Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method was used for the extraction of small bioorganic molecules from ground dried material. [Read More]

Consumption of the Sugar Substitute Stevia Leads to Cross-Reactivity of CEDIA(R) Buprenorphine II Immunoassay

Plattner,S.; Pavlic,M.; Pitterl,F.; Schubert,B.; Buprenorphine is a semisynthetic opioid that is often used in opiate maintenance therapy. For this purpose, regular toxicological analyses of urine samples are mandatory. For fast analytical results, analyses are commonly performed by immunoassay, for example, Thermo Scientific CEDIA® Buprenorphine or Buprenorphine II assay. One drawback of immunoassay-based methods is the possible cross-reaction with other substances. Several drugs have already been checked for cross-reactivity to CEDIA® Buprenorphine II immunoassay. [Read More]

Development and Evaluation of the Ancestry Informative Marker Panel of the VISAGE Basic Tool

delaPuente,M.; Ruiz-Ramirez,J.; Ambroa-Conde,A.; Xavier,C.; Pardo-Seco,J.; Alvarez-Dios,J.; Freire-Aradas,A.; Mosquera-Miguel,A.; Gross,T.E.; Cheung,E.Y.Y.; Branicki,W.; Nothnagel,M.; Parson,W.; Schneider,P.M.; Kayser,M.; Carracedo,A.; Lareu,M.V.; Phillips,C.; OnBehalfOfTheVisage,Consortium; We detail the development of the ancestry informative single nucleotide polymorphisms (SNPs) panel forming part of the VISAGE Basic Tool (BT), which combines 41 appearance predictive SNPs and 112 ancestry predictive SNPs (three SNPs shared between sets) in one massively parallel sequencing (MPS) multiplex, whereas blood-based age analysis using methylation markers is run in a parallel MPS analysis pipeline. [Read More]

Development and inter-laboratory validation of the VISAGE enhanced tool for age estimation from semen using quantitative DNA methylation analysis

Heidegger,A.; Pisarek,A.; delaPuente,M.; Niederstätter,H.; Pospiech,E.; Wozniak,A.; Schury,N.; Unterländer,M.; Sidstedt,M.; Junker,K.; VentayolGarcia,M.; Laurent,F.X.; Ulus,A.; Vannier,J.; Bastisch,I.; Hedman,J.; Sijen,T.; Branicki,W.; Xavier,C.; Parson,W.; VisageConsortium; The analysis of DNA methylation has become an established method for chronological age estimation. This has triggered interest in the forensic community to develop new methods for age estimation from biological crime scene material. Various assays are available for age estimation from somatic tissues, the majority from blood. Age prediction from semen requires different DNA methylation markers and the only assays currently developed for forensic analysis are based on SNaPshot or pyrosequencing. [Read More]

Development of the VISAGE enhanced tool and statistical models for epigenetic age estimation in blood, buccal cells and bones

Wozniak,A.; Heidegger,A.; Piniewska-Rog,D.; Pospiech,E.; Xavier,C.; Pisarek,A.; Kartasinska,E.; Boron,M.; Freire-Aradas,A.; Wojtas,M.; delaPuente,M.; Niederstätter,H.; Ploski,R.; Spolnicka,M.; Kayser,M.; Phillips,C.; Parson,W.; Branicki,W.; VisageConsortium; DNA methylation is known as a biomarker for age with applications in forensics. Here we describe the VISAGE (VISible Attributes through GEnomics) Consortium’s enhanced tool for epigenetic age estimation in somatic tissues. The tool is based on eight DNA methylation markers (44 CpGs), bisulfite multiplex PCR followed by sequencing on the MiSeq FGx platform, and three statistical prediction models for blood, buccal cells and bones. [Read More]