Brandstätter,A.; Parsons,T.J.; Parson,W.;
Progress in Forensic Genetics 10 2004
New sequence data of allelic variants at the STR loci ACTBP2 (SE33), D21S11, FGA, vWA, CSF1PO, D2S1338, D16S539, D18S51 and D19S433 in Caucasoids
Dauber,E.M.; Bär,W.; Klintschar,M.; Neuhuber,F.; Parson,W.; Mueller-van der Spruit,E.; Mayr,W.R.;
Progress in Forensic Genetics 10 2004
Optimized Suppression of Adducts in Polymerase Chain Reaction Products for Semi-Quantitative SNP Genotyping by Liquid Chromatography-Mass Spectrometry
Oberacher,H.; Parson,W.; Hölzl,G.; Oefner,P.J.; Huber,C.G.;
While electrospray ionization mass spectrometry has shown great potential for the identification of genotypes in DNA sequences amplified by polymerase chain reaction (PCR), the quantitative determination of allele frequencies remains challenging because of the presence of cationic adducts in the mass spectra which severely impairs accuracy of quantitation. We report here on the elaboration of an optimized desalting protocol for ion-pair reversed-phase high-performance liquid chromatography-electrospray ionization mass spectrometry (ICEMS) of PCR amplicons which facilitates the genotyping of single nucleotide polymorphisms (SNPs).
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Phylogenetic and genotypic resistance analysis of HIV-1 infected patients in Austria
Falkensammer,B.; Doerler,M.; Kessler,H.H.; Puchammer-Stoeckl,E.; Parson,W.; Taveira,N.;
BACKGROUND: Analysis of HIV-1 subtypes and genotypic resistance have been shown to be relevant for epidemiologic and therapeutic studies or for vaccine development. In Europe, the majority of HIV-1 isolates belong to subtype B. Due to migration an increasing incidence for additional subtypes and complex recombinant forms are expected. OBJECTIVES AND STUDY DESIGN: To evaluate the prevalence of HIV-1 subtypes in Austria, 188 plasma samples of treatment experienced patients were investigated.
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Results of a collaborative study of the EDNAP group regarding mitochondrial DNA heteroplasmy and segregation in hair shafts
Tully,G.; Barritt,S.M.; Bender,K.; Brignon,E.; Capelli,C.; mo-Simonin,N.; Eichmann,C.; Ernst,C.M.; Lambert,C.; Lareu,M.V.; Ludes,B.; Mevag,B.; Parson,W.; Pfeiffer,H.; Salas,A.; Schneider,P.M.; Staalstrom,E.;
A collaborative exercise was carried out by the European DNA Profiling Group (EDNAP) in order to evaluate the distribution of mitochondrial DNA (mtDNA) heteroplasmy amongst the hairs of an individual who displays point heteroplasmy in blood and buccal cells. A second aim of the exercise was to study reproducibility of mtDNA sequencing of hairs between laboratories using differing chemistries, further to the first mtDNA reproducibility study carried out by the EDNAP group.
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