Alcohol and/or benzodiazepine use in injured road users

 Published in 2003

Kurzthaler,I.; Wambacher,M.; Golser,K.; Sperner,G.; Sperner-Unterweger,B.; Haidekker,A.; Pavlic,M.; Kemmler,G.; Fleischhacker,W.W.; Blood samples of all patients (269) involved in a traffic accident and admitted to the Emergency Room of the University Hospital of Trauma Surgery in Innsbruck were analysed for alcohol and benzodiazepines. The large majority were drivers (55%) followed by passengers (19.7%), cyclists (12.6%) and pedestrians (12.3%). Alcohol was obviously the most commonly found drug in all groups (drivers: 36.9%; passengers: 15. [Read More]

Characterization of the Aspergillus nidulans transporters for the siderophores enterobactin and triacetylfusarinine C

 Published in 2003

Haas,H.; Schoeser,M.; Lesuisse,E.; Ernst,J.F.; Parson,W.; Abt,B.; Winkelmann,G.; Oberegger,H.; The filamentous ascomycete Aspergillus nidulans produces three major siderophores: fusigen, triacetylfusarinine C, and ferricrocin. Biosynthesis and uptake of iron from these siderophores, as well as from various heterologous siderophores, is repressed by iron and this regulation is mediated in part by the transcriptional repressor SREA. Recently we have characterized a putative siderophore-transporter-encoding gene ( mirA ). Here we present the characterization of two further SREA- and iron-regulated paralogues (mirB and mirC ), including the chromosomal localization and the complete exon/intron structure. [Read More]

Cloning Of An Emopamil-Binding Protein (Ebp)-Like Protein That Lacks Sterol Delta(8)-Delta(7) Isomerase Activity

 Published in 2003

Moebious,F.F.; Fitzky,B.U.; Wietzorrek,G.; Haidekker,A.; Eder,A.; Glossmann,H.; EBP (emopamil-binding protein) is a high-affinity binding protein for [3H]emopamil and belongs to the family of so-called sigma receptors. Mutations that disrupt EBP’s 3beta-hydroxysteroid sterol delta8-delta7 isomerase activity (EC 5.3.3.5) impair cholesterol biosynthesis and cause X-chromosomal dominant chondrodysplasia punctata. We identified a human cDNA for a novel EBPL (EBP-like protein) with a calculated mass of 23.2 kDa. Amino acid sequence alignments and phylogenetic analysis revealed that EBPL is distantly related to EBP (31% identity and 52% similarity) and found in animals but not in plants. [Read More]