Falkensammer,B.; El Attal,R.; Mullauer,B.; Sarcletti,M.; Parson,W.; Dierich,M.P.; Stoiber,H.; The standard treatment for HIV infected patients is the highly active antiretroviral therapy. Due to resistance developments treatment failure can be found in some patients. In our study two different strategies are compared, which may reduce resistance mutations. Six patients (group A and B) have been monitored for about six years. Group A patients had a switch in their AZT-containing treatment to non AZT-containing regimens. [Read More]

Berger,A.P.; Parson,W.; Stenzl,A.; Steiner,H.; Bartsch,G.; Klocker,H.; OBJECTIVES: Bladder cancer is the result of clonal expansion of cancer cells in which multiple genetic alterations have occurred. Loss of heterozygosity (LOH) studies have demonstrated that alterations in microsatellite regions are common in bladder cancer. This observation offers the possibility of early tumor detection by examining the DNA of urinary sediment.METHODS: We investigated alterations of 17 microsatellite loci in urinary bladder carcinomas of different stages and grades. [Read More]

Steinlechner,M.; Berger,B.; Niederstätter,H.; Parson,W.; Determination of sex using the amelogenin sex test is well established in the forensic field especially for casework and DNA databasing purposes. The sex test is part of commercially available PCR kits. Among 29,432 phenotypic male individuals stored in the Austrian National DNA database, 6 individuals were found to lack the amelogenin Y-specific PCR product which was confirmed using alternative amelogenin primers. The amplification of eight Y-chromosomal STR markers resulted in full profiles in five out of the six samples, one sample failed to amplify Y-STRs at all. [Read More]

Oberegger,H.; Schoeser,M.; Zadra,I.; Schrettl,M.; Parson,W.; Haas,H.; In the filamentous fungus Aspergillus nidulans, iron homeostasis is regulated at the transcriptional level by the negative-acting GATA factor SREA. In this study the expression of a putative heme-containing metalloreductase-encoding gene, freA, was found to be upregulated by iron limitation independently of SREA, demonstrating the existence of an iron-regulatory mechanism which does not involve SREA. In contrast to freA, various other genes encoding proteins in need of iron-containing cofactors-acoA, lysF, and cycA-were downregulated in response to iron depletion. [Read More]

Berger,B.; Holzl,G.; Oberacher,H.; Niederstätter,H.; Huber,C.G.; Parson,W.; A method is described for genotyping alleles of the Y-chromosomal locus M9, incorporating DNA extraction, amplification by polymerase chain reaction (PCR), sample purification by ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC), and allele identification by on-line hyphenation to electrospray ionization mass spectrometry (ESI-MS). The alleles G and C were differentiated in 114 base pair amplicons on the basis of intact molecular mass measurements with a mass accuracy between 0. [Read More]