STR loci Penta D and Penta E: Austrian Caucasian population data

 Published in 2002

Steinlechner,M.; Grubwieser,P.; Scheithauer,R.; Parson,W.;

Allele frequencies for the short tandem repeat (STR) loci Penta D and Penta E, included in the PowerPlex 16 kit were obtained from a sample of 269 unrelated Austrian Caucasian individuals. Both loci met Hardy-Weinberg expectations

Int J Legal Med 2002 116(3):174-175
PubMed: 12111322

Sudden infant death in Austria--status quo and recommendations of the SIDS Consensus Working Group for Improved Registration

 Published in 2002

Ipsiroglu,O.S.; Fatemi,A.; Rabl,W.; Klupp,N.; Roll,P.; Meyer,H.; Kerbl,R.; Kurz,R.; Sudden infant death (SID) is the most common cause of death among infants aged 2 to 12 months in Austria. The complete autopsy required in order to diagnose SID, including the additional investigations, and the comprehensive autopsies needed for epidemiological studies are not common practice because of the different statutory regulations in the provinces and the absence of a uniform federal law in this regard. [Read More]

Analysis of polymerase chain reaction products by on-line liquid chromatography-mass spectrometry for genotyping of polymorphic short tandem repeat loci

 Published in 2001

Oberacher,H.; Parson,W.; Mühlmann,R.; Huber,C.G.; Capillary ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC) was used to separate and purify DNA fragments amplified by the polymerase chain reaction (PCR) prior to their characterization by electrospray ionization mass spectrometry (ESI-MS). The investigation by ESI-MS of single- or double stranded species could be effortlessly selected by chromatography of the nucleic acids under either nondenaturing or denaturing conditions, which were realized by proper adjustment of the column temperature. [Read More]

Automation and high through-put for a DNA database laboratory: development of a laboratory information management system

 Published in 2001

Steinlechner,M.; Parson,W.; Automation and high through-put production of DNA profiles has become a necessity in every DNA database unit. In our laboratory we developed a Laboratory Information Management System (LIMS) controlled workflow architecture, which comprises a robotic DNA extraction- and pipetting-system and a capillary electrophoresis unit. This allows a through-put of 4,000 samples per person per year. Improved sample handling and data management, full sample- and batch-histories, and software-aided supervision of result data, with a consequent average turn-around time of 8 days, are the main features of our new system [Read More]