Expression in insect cells of the functional domain of CD21 (complement receptor type two) as a truncated soluble molecule using a baculovirus vector

Prodinger,W.M.; Schoch,J.; Schwendinger,M.G.; Hellwage,J.; Parson,W.; Zipfel,P.F.; Dierich,M.P.; We have made use of the plasmid vector pBSV-8His recently established for baculovirus-mediated expression of His-tagged proteins for the production of a truncated soluble complement regulator protein. The protein comprised the N-terminal part, i.e. short consensus repeats (SCRs) 1-4, of the B-cell membrane protein complement receptor type two (CR2; CD21) and contained the functional epitopes which mediate the binding of the complement component C3 fragments C3dg and iC3b. [Read More]

Report of the European DNA profiling group (EDNAP): an investigation of the complex STR loci D21S11 and HUMFIBRA (FGA)

Gill,P.; D’Aloja,E.; Andersen,J.; Dupuy,B.; Jangblad,M.; Johnsson,V.; Kloosterman,A.D.; Kratzer,A.; Lareu,M.V.; Meldegaard,M.; Phillips,C.; Pfitzinger,H.; Rand,S.; Sabatier,M.; Scheithauer,R.; Schmitter,H.; Schneider,P.; Vide,M.C.; This paper describes a collaborative exercise which was intended to demonstrate whether uniformity of DNA profiling results could be achieved between European laboratories using two complex short tandem repeat (STR) loci. The loci D21S11 and HUMFIBRA (FGA) were chosen because they are commonly used by different European laboratories. D21S11 has approximately 14 common alleles (f > 0. [Read More]

Review of active compression-decompression cardiopulmonary resuscitation (ACD-CPR). Analysis of iatrogenic complications and their biomechanical explanation

Rabl,W.; Baubin,M.; Haid,C.; Pfeiffer,K.P.; Scheithauer,R.; Our review takes a critical look at the active compression-decompression technique (ACD) for cardiopulmonary resuscitation (CPR). ACD-CPR was developed following a report of successful resuscitation performed by a medical amateur using a household plunger. The efficacy of the principle of active decompression has been demonstrated by animal and human studies. Potential iatrogenic complications from the CardioPump were evaluated only when large clinical trials were already underway. [Read More]

Biomechanical Properties of the Human Tibia - Fracture Behavior and Morphology

Rabl,W.; Haid,C.; Krismer,M.; Standardized biomechanical dynamic load tests were performed to obtain fundamental information on the fracture behavior and morphology of the human tibia. After preparation, the specimens (n = 32) were loaded to breakage by ventral (one side alternately), dorsal, medial or lateral loading on a servo-hydraulic testing machine (Walter und Bai, Löhningen, Switzerland). Primary and secondary fracture lines and fissures were marked differently on the three surfaces of the tibia specimens. [Read More]