DNA Testing Reveals the Putative Identity of JB55, a 19th Century Vampire Buried in Griswold, Connecticut

Daniels-Higginbotham,J.; Gorden,E.M.; Farmer,S.K.; Spatola,B.; Damann,F.; Bellantoni,N.; Gagnon,K.S.; delaPuente,M.; Xavier,C.; Walsh,S.; Parson,W.; McMahon,T.P.; Marshall,C.; In 1990 in Griswold, Connecticut, archaeologists excavated a burial found in a “skull and crossbones” orientation. The lid of the 19th century coffin had brass tacks that spelled “JB55”, the initials of the person lying there and age at death. JB55 had evidence of chronic pulmonary infection, perhaps tuberculosis. It is possible that JB55 was deemed a vampire due to his disease, and therefore had to be “killed” by mutilating his corpse. [Read More]

Evaluation of mitogenome sequence concordance, heteroplasmy detection, and haplogrouping in a worldwide lineage study using the Precision ID mtDNA Whole Genome Panel

Strobl,C.; ChurchillCihlar,J.; Lagace,R.; Wootton,S.; Roth,C.; Huber,N.; Schnaller,L.; Zimmermann,B.; Huber,G.; LayHong,S.; Moura-Neto,R.; Silva,R.; Alshamali,F.; Souto,L.; Anslinger,K.; Egyed,B.; Jankova-Ajanovska,R.; Casas-Vargas,A.; Usaquen,W.; Silva,D.; Barletta-Carrillo,C.; Tineo,D.H.; Vullo,C.; Wurzner,R.; Xavier,C.; Gusmao,L.; Niederstatter,H.; Bodner,M.; Budowle,B.; Parson,W.; The emergence of Massively Parallel Sequencing technologies enabled the analysis of full mitochondrial (mt)DNA sequences from forensically relevant samples that have, so far, only been typed in the control region or its hypervariable segments. In this study, we evaluated the performance of a commercially available multiplex-PCR-based assay, the Precision ID mtDNA Whole Genome Panel (Thermo Fisher Scientific), for the amplification and sequencing of the entire mitochondrial genome (mitogenome) from even degraded forensic specimens. [Read More]

Forensic characterization and statistical considerations of the CaDNAP 13-STR panel in 1,184 domestic dogs from Germany, Austria, and Switzerland

Berger,B.; Heinrich,J.; Niederstatter,H.; Hecht,W.; Morf,N.; Hellmann,A.; Rohleder,U.; Schleenbecker,U.; Berger,C.; Parson,W.; Ca,DnapGroup; Crime scene samples originating from domestic dogs such as hair, blood, or saliva can be probative as possible transfer evidence in human crime and in dog attack cases. In the majority of such cases canine DNA identification using short tandem repeat (STR) analysis is the method of choice, which demands, among others, a systematic survey of allele frequency data in the relevant dog populations. [Read More]

MAPlex - A massively parallel sequencing ancestry analysis multiplex for Asia-Pacific populations

Phillips,C.; McNevin,D.; Kidd,K.K.; Lagace,R.; Wootton,S.; delaPuente,M.; Freire-Aradas,A.; Mosquera-Miguel,A.; Eduardoff,M.; Gross,T.; Dagostino,L.; Power,D.; Olson,S.; Hashiyada,M.; Oz,C.; Parson,W.; Schneider,P.M.; Lareu,M.V.; Daniel,R.; Current forensic ancestry-informative panels are limited in their ability to differentiate populations in the Asia-Pacific region. MAPlex (Multiplex for the Asia-Pacific), a massively parallel sequencing (MPS) assay, was developed to improve differentiation of East Asian, South Asian and Near Oceanian populations found in the extensive cross-continental Asian region that shows complex patterns of admixture at its margins. [Read More]

Massively parallel sequence data of 31 autosomal STR loci from 496 Spanish individuals revealed concordance with CE-STR technology and enhanced discrimination power

Barrio,P.A.; Martin,P.; Alonso,A.; Muller,P.; Bodner,M.; Berger,B.; Parson,W.; Budowle,B.; DnaseqexConsortium; This study reports Short Tandem Repeat (STR) sequence-based allele data from 496 Spanish individuals across 31 autosomal STR (auSTR) loci included in the Precision ID GlobalFiler NGS STR Panel v2: D12S391, D13S317, D8S1179, D21S11, D3S1358, D5S818, D1S1656, D2S1338, vWA, D2S441, D5S2800, D7S820, D16S539, D6S474, D12ATA63, D4S2408, D6S1043, D19S433, D14S1434, CSF1PO, D10S1248, D18S51, D1S1677, D22S1045, D2S1776, D3S4529, FGA, Penta D, Penta E, TH01 and TPOX. [Read More]