Oberacher,H.; Pitterl,F.; Chervet,J.P.; Redox reactions are integral parts of many cellular processes. They are therefore extensively studied in vitro and in vivo. Electrochemistry (EC) represents a purely instrumental approach to characterize direct and indirect effects of redox reactions on bioorganic molecules, such as peptides, proteins, and endogenous and exogenous small molecules as well as nucleic acids. In addition to direct infusion electrospray ionization (ESI)-mass spectrometry (MS), hyphenated techniques such as liquid chromatography-mass spectrometry (LC-MS) are often applied for comprehensive characterization of reaction mixtures generated by EC experiments. [Read More]

Longato,S.; Woss,C.; Hatzer-Grubwieser,P.; Bauer,C.; Parson,W.; Unterberger,S.H.; Kuhn,V.; Pemberger,N.; Pallua,A.K.; Recheis,W.; Lackner,R.; Stalder,R.; Pallua,J.D.; In this study different state-of-the-art visualization methods such as micro-computed tomography (micro-CT), mid-infrared (MIR) microscopic imaging and energy dispersive X-ray (EDS) mapping were evaluated to study human skeletal remains for the determination of the post-mortem interval (PMI). PMI specific features were identified and visualized by overlaying molecular imaging data and morphological tissue structures generated by radiological techniques and microscopic images gained from confocal microscopy (Infinite Focus (IFM)). [Read More]

Haas,C.; Hanson,E.; Banemann,R.; Bento,A.M.; Berti,A.; Carracedo,A.; Courts,C.; DeCock,G.; Drobnic,K.; Fleming,R.; Franchi,C.; Gomes,I.; Hadzic,G.; Harbison,S.A.; Hjort,B.; Hollard,C.; Hoff-Olsen,P.; Keyser,C.; Kondili,A.; Maronas,O.; McCallum,N.; Miniati,P.; Morling,N.; Niederstatter,H.; Noel,F.; Parson,W.; Porto,M.J.; Roeder,A.D.; Sauer,E.; Schneider,P.M.; Shanthan,G.; Sijen,T.; SyndercombeCourt,D.; Turanska,M.; vandenBerge,M.; Vennemann,M.; Vidaki,A.; Zatkalikova,L.; Ballantyne,J.; The European DNA profiling group (EDNAP) organized a sixth collaborative exercise on RNA/DNA co-analysis for body fluid/tissue identification and STR profiling. The task was to identify skin samples/contact traces using specific RNA biomarkers and test three housekeeping genes for their suitability as reference genes. [Read More]

vandenBerge,M.; Carracedo,A.; Gomes,I.; Graham,E.A.; Haas,C.; Hjort,B.; Hoff-Olsen,P.; Maronas,O.; Mevag,B.; Morling,N.; Niederstätter,H.; Parson,W.; Schneider,P.M.; Court,D.S.; Vidaki,A.; Sijen,T.; The European Forensic Genetics Network of Excellence (EUROFORGEN-NoE) undertook a collaborative project on mRNA-based body fluid/skin typing and the interpretation of the resulting RNA and DNA data. Although both body fluids and skin are composed of a variety of cell types with different functions and gene expression profiles, we refer to the procedure as ‘cell type inference’. [Read More]

Purps,J.; Siegert,S.; Willuweit,S.; Nagy,M.; Alves,C.; Salazar,R.; Angustia,S.M.; Santos,L.H.; Anslinger,K.; Bayer,B.; Ayub,Q.; Wei,W.; Xue,Y.; Tyler-Smith,C.; Bafalluy,M.B.; Martinez-Jarreta,B.; Egyed,B.; Balitzki,B.; Tschumi,S.; Ballard,D.; Court,D.S.; Barrantes,X.; Bassler,G.; Wiest,T.; Berger,B.; Niederstätter,H.; Parson,W.; Davis,C.; Budowle,B.; Burri,H.; Borer,U.; Koller,C.; Carvalho,E.F.; Domingues,P.M.; Chamoun,W.T.; Coble,M.D.; Hill,C.R.; Corach,D.; Caputo,M.; D’Amato,M.E.; Davison,S.; Decorte,R.; Larmuseau,M.H.; Ottoni,C.; Rickards,O.; Lu,D.; Jiang,C.; Dobosz,T.; Jonkisz,A.; Frank,W.E.; Furac,I.; Gehrig,C.; Castella,V.; Grskovic,B.; Haas,C.; Wobst,J.; Hadzic,G.; Drobnic,K.; Honda,K.; Hou,Y.; Zhou,D.; Li,Y.; Hu,S.; Chen,S.; Immel,U.D.; Lessig,R.; Jakovski,Z.; Ilievska,T.; Klann,A.E.; Garcia,C.C.; deKnijff,P. [Read More]