Oberacher,H.; Whitley,G.; Berger,B.; Weinmann,W.; A tandem mass spectral database system consists of a library of reference spectra and a search program. State-of-the-art search programs show a high tolerance for variability in compound-specific fragmentation patterns produced by collision-induced decomposition and enable sensitive and specific ‘identity search’. In this communication, performance characteristics of two search algorithms combined with the ‘Wiley Registry of Tandem Mass Spectral Data, MSforID’ (Wiley Registry MSMS, John Wiley and Sons, Hoboken, NJ, USA) were evaluated. [Read More]

Ausserlechner,M.J.; Salvador,C.; Deutschmann,A.; Bodner,M.; Viola,G.; Bortolozzi,R.; Basso,G.; Hagenbuchner,J.; Obexer,P.; Forkhead transcription factors (FOXO) are downstream targets of the phosphoinositol-3-kinase (PI3K) protein kinase B (PKB) signaling cascade and play a pivotal role in cell differentiation, cell cycle and apoptosis. We found that cells from prednisone-resistant T-acute lymphoblastic leukemia (T-ALL) patients showed cytoplasmic localization of FOXO3 in comparison to prednisone-sensitive patients suggesting its inactivation. To determine the impact of FOXO3, T-ALL cells were infected with a 4OH-tamoxifen-regulated, phosphorylation-independent FOXO3(A3)ERtm allele. [Read More]

Salas,A.; Coble,M.; Desmyter,S.; Grzybowski,T.; Gusmao,L.; Hohoff,C.; Holland,M.M.; Irwin,J.A.; Kupiec,T.; Lee,H.Y.; Ludes,B.; Lutz-Bonengel,S.; Melton,T.; Parsons,T.J.; Pfeiffer,H.; Prieto,L.; Tagliabracci,A.; Parson,W.;

Forensic Sci.Int Genet. 2012 6(6):e182-e184
PubMed: 22840856

Beer,B.; Krjutskov,K.; Erb,R.; Viltrop,T.; Oberacher,H.; Among numerous available genotyping techniques, mass spectrometry (MS) based methods play a major role in providing high quality genotype data at reasonable costs for research and diagnostics, e.g. for pharmacogenetic applications. Ion-pair reversed-phase liquid chromatography hyphenated to electrospray ionization time-of-flight MS (ICEMS) is, for example, a powerful instrument that allows a direct characterization of complex mixtures of polymerase chain reaction (PCR) amplified DNA fragments. Current limitations of PCR-ICEMS genotyping are mainly concerned with the multiplex PCR set-up. [Read More]

Oberguggenberger,A.S.; Sztankay,M.; Beer,B.; Schubert,B.; Meraner,V.; Oberacher,H.; Kemmler,G.; Giesinger,J.; Gamper,E.; Sperner-Unterweger,B.; Marth,C.; Holzner,B.; Hubalek,M.; ABSTRACT: BACKGROUND: Current studies on adherence to endocrine therapy in breast cancer patients suffer from methodological limitations due to a lack of well-validated methods for assessing adherence. There is no gold standard for measuring adherence. The aim of our study was to compare four different approaches for evaluating adherence to anastrozole therapy for breast cancer with regard to concordance between methods. [Read More]