Hatzer-Grubwieser,P.; Berger,B.; Niederwieser,D.; Steinlechner,M.;
Forensic Sci.Int Genet. 2012 6(1):e50-e51
PubMed: 21531186
An optimized electrochemistry-liquid chromatography-mass spectrometry method for studying guanosine oxidation
Erb,R.; Plattner,S.; Pitterl,F.; Brouwer,H.J.; Oberacher,H.;
Oxidative stress can disrupt the integrity of genetic material. Due to its importance in the pathogenesis of different kinds of disease, including neurodegenerative disease, cardiovascular disease and cancer, major efforts are put into the elucidation of mechanisms involved. Herein, the combination of electrochemistry/liquid chromatography/mass spectrometry (EC/LC/MS) is presented as convenient, fast and simple method to study nucleic acids oxidation. Guanosine was selected as test compound. 8-Hydroxyguanosine and (guanosine-H)(2) were identified as primary oxidation products.
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Arrival of paleo-indians to the southern cone of South america: new clues from mitogenomes
de Saint,Pierre M.; Gandini,F.; Perego,U.A.; Bodner,M.; Gomez-Carballa,A.; Corach,D.; Angerhofer,N.; Woodward,S.R.; Semino,O.; Salas,A.; Parson,W.; Moraga,M.; Achilli,A.; Torroni,A.; Olivieri,A.;
WITH ANALYSES OF ENTIRE MITOGENOMES, STUDIES OF NATIVE AMERICAN MITOCHONDRIAL DNA (MTDNA) VARIATION HAVE ENTERED THE FINAL PHASE OF PHYLOGENETIC REFINEMENT: the dissection of the founding haplogroups into clades that arose in America during and after human arrival and spread. Ages and geographic distributions of these clades could provide novel clues on the colonization processes of the different regions of the double continent.
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Ascorbic acid for homogenous redox buffering in electrospray ionization-mass spectrometry
Plattner,S.; Erb,R.; Chervet,J.P.; Oberacher,H.;
Electrospray ionization (ESI) involves the dispersion of a liquid containing analytes of interest into a fine aerosol by applying a high potential difference to the sample solution with respect to a counter electrode. Thus, from the electrochemical point of view, the ESI source represents a two-electrode controlled-current electrochemical flow cell. The electroactive compounds part of the solvent sprayed may be altered by occurring electrolysis (oxidation in positive ion mode and reduction in negative ion mode).
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DNA commission of the International Society of Forensic Genetics: Recommendations on the evaluation of STR typing results that may include drop-out and/or drop-in using probabilistic methods
Gill,P.; Gusmao,L.; Haned,H.; Mayr,W.R.; Morling,N.; Parson,W.; Prieto,L.; Prinz,M.; Schneider,H.; Schneider,P.M.; Weir,B.S.;
DNA profiling of biological material from scenes of crimes is often complicated because the amount of DNA is limited and the quality of the DNA may be compromised. Furthermore, the sensitivity of STR typing kits has been continuously improved to detect low level DNA traces. This may lead to (1) partial DNA profiles and (2) detection of additional alleles. There are two key phenomena to consider: allelic or locus ‘drop-out’, i.
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