Berger,C.; Berger,B.; Parson,W.; In recent years, evidence from domestic dogs has increasingly been analyzed by forensic DNA testing. Especially, canine hairs have proved most suitable and practical due to the high rate of hair transfer occurring between dogs and humans. Starting with the description of a contamination-free sample handling procedure, we give a detailed workflow for sequencing hypervariable segments (HVS) of the mtDNA control region from canine evidence. After the hair material is lysed and the DNA extracted by Phenol/Chloroform, the amplification and sequencing strategy comprises the HVS I and II of the canine control region and is optimized for DNA of medium-to-low quality and quantity. [Read More]

Wolfram,D.; Rabensteiner,E.; Grundtman,C.; Böck,G.; Mayerl,C.; Parson,W.; Almanzar,G.; Hasenöhrl,C.; Piza-Katzer,H.; Wick,G.; BACKGROUND: : The authors investigated the immunological mechanisms underlying extensive peri-silicone implant capsule formation, one of the most frequent postoperative complications in patients receiving silicone mammary implants. METHODS: : The authors studied immune response activation by phenotypic and functional characterization of lymphocytes accumulated within this tissue. Intracapsular lymphoid cells and autologous peripheral blood mononuclear cells were isolated and analyzed by flow cytometry. [Read More]

Herndler-Brandstetter,D.; Landgraf,K.; Tzankov,A.; Jenewein,B.; Brunauer,R.; Laschober,G.T.; Parson,W.; Kloss,F.; Gassner,R.; Lepperdinger,G.; Grubeck-Loebenstein,B.; Recently, the BM has been shown to play a key role in regulating the survival and function of memory T cells. However, the impact of aging on these processes has not yet been studied. We demonstrate that the number of CD4(+) and CD8(+) T cells in the BM is maintained during aging. However, the composition of the T cell pool in the aged BM is altered with a decline of naive and an increase in T(EM) cells. [Read More]

Welch,L.; Gill,P.; Tucker,V.C.; Schneider,P.M.; Parson,W.; Mogensen,H.S.; Morling,N.;

Forensic Sci.Int Genet. 2011 5(3):257-258
PubMed: 20457074

Fendt,L.; Niederstätter,H.; Huber,G.; Zelger,B.; Dünser,M.; Seifarth,C.; Röck,A.; Schäfer,G.; Klocker,H.; Parson,W.; The occurrence of heteroplasmy and mixtures is technically challenging for the analysis of mitochondrial DNA. More than that, observed mutations need to be carefully interpreted in the light of the phylogeny as mitochondrial DNA is a uniparental marker reflecting human evolution. Earlier attempts to explain the role of mtDNA in cancerous tissues led to substantial confusion in medical genetics mainly due to the presentation of low sequence data quality and misinterpretation of mutations representing a particular haplogroup background rather than being cancer-specific. [Read More]