Pitterl,F.; Chervet,J.P.; Oberacher,H.; Oxidation is commonly involved in the alteration of nucleic acids giving rise to diverse effects including mutation, cell death, malignancy, and aging. We demonstrate that electrochemistry represents an efficient and fast method to mimic oxidative modification of nucleic acids occurring in biological systems. Oxidation reactions were performed in a thin-layer cell employing a conductive diamond electrode as the working electrode and were monitored with electrospray ionization-mass spectrometry. Mass voltammograms were acquired for guanosine, adenosine, cytidine, and uridine. [Read More]

Herff,H.; Loosen,S.J.; Paal,P.; Mitterlechner,T.; Rabl,W.; Wenzel,V.; Apart from misdiagnosis, the Lazarus phenomenon, a spontaneous return of circulation after cardiac arrest, is a potential cause for false positive death certification. Because of medicolegal consequences and thus a negative publication bias, the incidence of false positive death certification is unknown. As a false positive death certification results in criminal prosecution and thus media interest, numerous media archives in Germany, Austria and Switzerland were searched for such reports. [Read More]

Oberacher,H.; Nucleic acids research is a highly competitive field of research. A number of well established methods are available. The current output of high throughput (“next generation”) sequencing technologies is impressive, and still technologies are continuing to make progress regarding read lengths, bp per second, accuracy and costs. Although in the 1990s MS was considered as an analytical platform for sequencing, it was soon realized that MS will never be competitive. [Read More]

Lessig,R.; Edelmann,J.; Aspinall,L.; Krumm,P.; Bastisch,I.; Wiegand,P.; Hohoff,C.; Steinlechner,M.; Roewer,L.;

Forensic Sci Int Genet 2010
PubMed: 20457065

Crubezy,E.; Amory,S.; Keyser,C.; Bouakaze,C.; Bodner,M.; Gibert,M.; Röck,A.; Parson,W.; Alexeev,A.; Ludes,B.; ABSTRACT: BACKGROUND: The Yakuts contrast strikingly with other populations from Siberia due to their cattle- and horse-breeding economy as well as their Turkic language. On the basis of ethnological and linguistic criteria as well as population genetic studies, it has been assumed that they originated from South Siberian populations. However, many questions regarding the origins of this intriguing population still need to be clarified (e. [Read More]