Subtype and genotypic resistance analysis of HIV-1 infected patients in Austria

Falkensammer,B.; Doerler,M.; Kessler,H.H.; Puchhammer-Stoeckl,E.; Parson,W.; Duftner,C.; Dierich,M.P.; Stoiber,H.; BACKGROUND: Analysis of HIV-1 subtypes and genotypic resistance have been shown to be relevant for epidemiologic and therapeutic studies or for vaccine development. In Europe, the majority of HIV-1 isolates belong to subtype B. Due to migration an increasing incidence for additional subtypes and complex recombinant forms are expected. OBJECTIVES AND STUDY DESIGN: To evaluate the prevalence of HIV-1 subtypes in Austria, 188 plasma samples of treatment experienced patients were investigated. [Read More]

Unravelling the mystery of Nanga Parbat

Parson,W.; Brandstätter,A.; Niederstätter,H.; Grubwieser,P.; Scheithauer,R.; Since the disappearance of Gunther Messner, brother of the world-famous mountaineer Reinhold Messner, on an expedition to Nanga Parbat in 1970, the circumstances of his death have given rise to controversy. Reinhold Messner claimed that he and Gunther descended the Diamir face together when an avalanche killed his brother, while other expedition members argued that Gunther was abandoned by Reinhold to descend the Rupal face. Now, 35 years after the event, Gunther’s remains have been found at the Diamir face and have been identified by forensic DNA fingerprinting. [Read More]

A new miniSTR-multiplex displaying reduced amplicon lengths for the analysis of degraded DNA

Grubwieser,P.; Mühlmann,R.; Berger,B.; Niederstätter,H.; Pavlic,M.; Parson,W.; A multiplex PCR was designed for the loci D2S1338, D16S539, D18S51, TH01 and FGA using redesigned primers in order to reduce the lengths of the amplification products compared to the designs used in commercially available multiplex PCR kits, also including amelogenin. The new PCR primers were used to amplify highly degraded DNA from casework samples, which had shown no or only poor results for these loci in previous analyses with standard primer sets. [Read More]

A proposal for standardization in forensic canine DNA typing: allele nomenclature of six canine-specific STR loci

Hellmann,A.P.; Rohleder,U.; Eichmann,C.; Pfeiffer,I.; Parson,W.; Schleenbecker,U.; In this study a proposal for the allele nomenclature of six polymorphic short tandem repeat (STR) loci (PEZ3, PEZ6, PEZ8, PEZ10, FHC2161, and FHC2328) for canine genotyping (Canis lupus familiaris) is presented. The nomenclature is based on the sequence data of the polymorphic region of the microsatellite markers as recommended by the DNA commission of the International Society of Forensic Haemogenetics (ISFH) in 1994 for human DNA typing. [Read More]