Some guidelines for the analysis of genomic DNA by PCR-LC-ESI-MS

Oberacher,H.; Niederstätter,H.; Casetta,B.; Parson,W.; Ion-pair reversed-phase high-performance liquid chromatography online hyphenated to electrospray ionization mass spectrometry (ICEMS) represents an efficient method for the characterization of nucleic acids amplified by polymerase chain reaction (PCR). Since sample preparation is limited to PCR, the optimization of its solution conditions is of utmost importance for efficient mass spectrometric detection. The compatibility of a number of different commercially available PCR components including DNA polymerases, deoxynucleotide triphosphates, bovine serum albumin, enhancer, and ionic buffers was evaluated. [Read More]

TSUNAMI-Disaster: DNA-Typing of Sri Lanka victim samples and related AM matching procedures

Steinlechner,M.; Parson,W.; Rabl,W.; Grubwieser,P.; Scheithauer,R.; More than 400 samples related to 101 tsunami victims from Sri Lanka were sent to our laboratory. Bar-coded sampling kits were used to collect two swabs from intact inner surfaces, one 4 cm piece of long bone and two teeth, which were all shipped on ice. A high throughput DNA extraction and STR analysis procedure for the swabs were developed in order to provide STR profiles within 24 hours. [Read More]