Forensic validation of the Precision ID mtDNA Whole Genome Panel Using the Ion Chef and the Ion S5 System

Strobl,C.; Schnaller,L.; Bodner,M.; Huber,G.; Zimmermann,B.; Chang,J.; Wootton,S.; Lagacé,R.; Parson,W.

In challenging cases where nuclear DNA markers fail to give adequate results, mitochondrial DNA can provide useful information especially when mitogenome sequencing is performed. Such degraded samples require the use of short amplicons, which can be achieved using the Precision ID mtDNA Whole Genome Panel (Thermo Fisher Scientific, CA). This kit allows sequencing the entire mitogenome using 162 amplicons with a maximum length of 175bp. The following study presents initial results from the developmental validation of the Precision ID kit processed with the Ion Chef Instrument and the Ion S5 System according to the manufacturer’s recommended protocol.