Systematic Evaluation of Massively Parallel STR Sequencing

Systematic Evaluation of Massively Parallel STR Sequencing in the DNASeqEx Project

Müller,P.; Berger,B.; Bodner,M.; Alonso,A.; Barrio,P.; Martin,P.; Köcher,S.; Roewer,L.; Willuweit,S.; Budowle,B.; DNASEQEX Consortium; Parson,W.

The EU funded DNASeqEx (DNA-STR Massive Sequencing & International Information Exchange) project evaluates current massively parallel sequencing (MPS) technologies in a collaborative manner. The aim of the project is to perform interlaboratory evaluation and comparison of forensically relevant parameters, such as allele calling, sensitivity, reproducibility, and concordance in STR analysis using single source and mixture samples.

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Tracing Arawakan Southern Dispersal

Tracing Arawakan Southern Dispersal: Clues from Mitogenome Sequencing of Southern Amerinds

Xavier,C.; Simão,F.; Bodner,M.; Strobl,C.; Silva,D.; Barletta,C.; Casas-Vargas,A.; Usaquén,W.; Tineo,D.H.; Gusmão,L.; Parson,W.

South America’s colonization and internal migration routes remain unsolved issues for the scientiic community. The wide variety of different environmental settings and the amplitude of climate variations at the time of colonization might explain the big variation of different tribes and groups in South America, in contrast to it’s northern counterpart.

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Y-chromosomal composition of mediaeval and contemporary populations in Norwa

Y-chromosomal composition of mediaeval and contemporary populations in Norway and adjacent Scandinavian countries: Y-STR haplotypes and the rare Y-haplogroup Q

Berger,B.; Willuweit,S.; Niederstätter,H.; Kralj,P.; Roewer,L.; Hamre,S.; Parson,W.

The project “Immigration and mobility in mediaeval and post-mediaeval Norway” is an international multidisciplinary research collaboration and is part of the Norwegian Research Council ́s ten-year research programme “SAMKUL” (Cultural conditions underlying social change). It aims to combine historical information with archeological, osteological, isotopic and genetic data obtained from medieval (Norway-M) and post-medieval (Norway-PM) skeletal remains excavated in Trondheim, one of the medieval capitals of Norway.

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Comparison of CEDIA Buprenorphine Assay

Comparison of CEDIA Buprenorphine II Assay vs. on Market CEDIA Buprenorphine Assay in Human Urine

Buprenorphine is a semi-synthetic opioid derived from the opioid alkaloid thebaine. It is widely used clinically as an analgesic and as a substitute therapy for opioid dependence. It is considered a relatively safe opioid because of its agonistic-antagonistic characteristics on opiate receptors Correlation between dose and therapeutic effects are not linear, indicating a ceiling on the effects in patients.

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Full mt-Genome Sequencing with mitochondrail Tiling Path Primers

Full mt-Genome Sequencing with mitochondrail Tiling Path Primers using the Ion Torrent PGM

Strobl,C.; Lagacé,R.; Chang,J.; Parson,W.

Sequencing analysis of mitochondrial DNA (mtDNA) has proved particularly important in forensic applications addressing samples that are not amenable to the typing of highly polymorphic nuclear DNA markers. However, sequencing of only the non-coding control region is sometimes not sufficiently informative for acquiring the required discrimination power needed in some forensic investigations. With the availability of new sequencing technologies, analysis of the complete mitochondrial genome (mtGenome) has become feasible and is likely to evolve into a method of first choice. For challenging samples routinely encountered in forensic casework, such as hair shafts or teeth and bones, which suffer severe environmental stress, DNA quantity and/or quality sometimes is insufficient for conventional Sanger sequencing. The purpose of this study was to test the utility of an alternative technique, the so called mito tiling approach, relying on massively parallel (next generation) sequencing, to sequence the entire human mtGenome in 162 amplicons of about 175bp in length .

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