An alternative strategy for whole mitochondrial genome amplification and sequencing suited for lower quality mtDNA

Zimmermann,B.; Huber,G.; Fendt,L.; Parson,W.

Amplification and sequencing of the entire mitochondrial (mt) genome becomes increasingly important in the fields of forensic DNA testing and phylogenetics, as control region sequencing and targeted coding region SNP typing are sometimes not enough for a clear haplogroup assignment. Nowadays the amplification and sequencing of the mtDNA control region (CR) seems to be a routine exercise in some labs, while sequencing of the whole mt genome (~16.6kb) is more demanding, especially when the DNA quality of the samples of interest is low.